Culturing P. falciparum in vitro |
– Basic Culture Maintenance |
– Making a Smear of Malaria Culture |
– Making Media for Culture | |
– Washing Blood |
– Culture Synchronization |
– Thawing Malaria Culture |
– Freezing Malaria Strains |
– SYBR Green Growth Assay |
Transfection |
– Transfection of P. falciparum |
Metabolomics Protocols | |
– Testing for Mycoplasma (CRITICAL) |
– Metabolite Extraction for LC/MS (short version) |
– Metabolite Extraction for LC/MS (detailed version) |
– Media Extraction for LC/MS |
Labeling of RNA with 4-thiouracil |
– RNA Analysis by Biosynthetic Labeling |
Nucleic Acid Isolation |
– Malaria Total RNA Isolation Trizol |
– DNA Miniprep for Cloning or Transfections |
– Genomic DNA isolation | |
– Genomic DNA isolation (DNeasy) |
Cloning |
– Ilsa’s Guide to Cloning |
PCR Procedures | |
– Colony PCR | |
– Polymerase Chain Reaction for P. falciparum | |
Protein Procedures | |
– Isolation of P. falciparum proteins (Saponin lysis) | |
– Isolation of P. falciparum nuclear proteins | |
– Purification of GST-fused proteins | |
– Tricine Polyacrylamide Protein Gel | |
– Laemmli Protein Gel | |
– Silver staining protein gels | |
– Electrophoretic Mobility Shift Assays (Gel-shifts) | |
Microarray Procedures | |
– RT and Amino-allyl Incorporation | |
– Genomic DNA Labeling | |
– NEW: cDNA Labeling for Agilent Arrays | |
– NEW: Hybridization and Washing for Agilent Arrays | |
– cDNA Labeling Hybridization and Washing | |
– Malaria Microarray Post Processing | |
– NEW: Protein Binding Microarray (PBM) Double Stranding | |
– NEW: Protein Binding Microarray (PBM) Hybing and Scanning | |
Making Chemically Competent E. Coli Cells | |