Protocols

Protocols

Culturing P. falciparum in vitro
- Basic Culture Maintenance Bioreactor
- Making a Smear of Malaria Culture
- Making Media for Culture
- Washing Blood
- Culture Synchronization
- Thawing Malaria Culture
- Freezing Malaria Strains
- SYBR Green Growth Assay
        Lab notebook
Transfection
- Transfection of P. falciparum
Metabolomics Protocols
- Testing for Mycoplasma (CRITICAL)
- Metabolite Extraction for LC/MS (short version)
- Metabolite Extraction for LC/MS (detailed version)
- Media Extraction for LC/MS
Labeling of RNA with 4-thiouracil
- RNA Analysis by Biosynthetic Labeling
Nucleic Acid Isolation        Microarray
- Malaria Total RNA Isolation Trizol
- DNA Miniprep for Cloning or Transfections
- Genomic DNA isolation
- Genomic DNA isolation (DNeasy)
Cloning
- Ilsa’s Guide to Cloning
PCR Procedures
- Colony PCR
- Polymerase Chain Reaction for P. falciparum
Protein Procedures
- Isolation of P. falciparum proteins (Saponin lysis)
- Isolation of P. falciparum nuclear proteins
- Purification of GST-fused proteins
- Tricine Polyacrylamide Protein Gel
- Laemmli Protein Gel
- Silver staining protein gels
- Electrophoretic Mobility Shift Assays (Gel-shifts)
Microarray Procedures
- RT and Amino-allyl Incorporation
- Genomic DNA Labeling
- NEW: cDNA Labeling for Agilent Arrays
- NEW: Hybridization and Washing for Agilent Arrays
- cDNA Labeling Hybridization and Washing
- Malaria Microarray Post Processing
- NEW: Protein Binding Microarray (PBM) Double Stranding
- NEW: Protein Binding Microarray (PBM) Hybing and Scanning
Making Chemically Competent E. Coli Cells